since cell damages occurs through this membrane damage. The n-Ag3O4 nanoparticles exhibit excellent antibacterial and antifungal effect compared to f-Ag3O4 nanoparticles and AMB. AMB exhibit excellent antibacterial effect against gram negative bacteria P. aeruginosa with MIC value of 20 ml and moderate antifungal effect against A. niger with MIC value of
80 ml, whereas it does not show any antibacterial effect against gram positive bacteria S. aureus even at higher concentration of 80 ml.