The optimum process variables that supported maximum phytase production by a marine yeast KodamaeaohmeriG3 were statistically determined as 23.0% NaCl and initial pH 6.3 [25]. [5] noticed that the growth of the food spoilage yeast D. nepalensis in NaCl and KCl was completely inhibited when temperature was maintained at extreme conditions (-1 and +1). At extreme pH of 3.6, specific growth was 0.29 and 0.37/h for NaCl and KCl, respectively exhibiting the yeast’s preference for acidic conditions for growth. Optimization of the process parameters using RSM clearly showed the strong interaction effect between the pH, temperature, and salt concentration on the growth of food spoilage yeast D. nepalensis.