Amplification of 12S rRNA was performed using two primers; SR-J-14233(F) and SR-N-14588 (R); targeting a 355 bp. Meanwhile, 16S rRNA was performed using two primers; LR-J-12887 (F) and LR-N-13398 (R); targeting a 511 bp. The universal primers namely LCO1490 (F), HCO2198 (R); targeting a 710 bp and specific primer namely C1-J-2195(F), TL2-N-3014 (R); targeting a 819 bp were amplified for cytochrome oxidase I gene. Primer information was summarized in Table2.