A decision support system specifically used to analyze the risk factors and predict the risk levels due to prolonged standing at work and to propose recommendations to minimize discomfort and fatigue has been developed, This study identified working body vibration, and indoor air quality as the risk factors for discomfort and muscle fatigue while workers are performing jobs in a standing position. The risk levels of the risk factors can be predicted through the PSSI value. The risk factors and the PSSI value have been modeled in a decision support system to provide systematic analysis and solutions for the discomfort and muscle fatigue associated with prolonged standing. Based on the results of the validation process, this study concluded that the decision support system is reliable for assessing, analyzing, and proposing solutions to minimize the discomfort and muscle fatigue associated with prolonged standing in industrial workplaces. This study suggests further efforts such as extensive testing and the development of an attractive and user-friendly GUI to make the decision support system commercially available.