The total NAD level in the
culture grown in the presence of ethanol was 3.7-fold higher than
that in the control (1.01 pmol/mg FW). This demonstrated that the
culture grown in the presence of ethanol was metabolically more
active than the culture grown in the absence of ethanol (as control).
This further substantiated the role of ethanol as a carbon
and energy source, as was evident from the substrate (glucose
and ethanol) consumption pattern described above in the presence
and absence of ethanol in the medium.