2 ml of lympholysis
buffer (50 mM EDTA pH 8.0, 50 mM Tris–HCl pH
7.5, 150 mM NaCl, 10% SDS) and 125 ll of proteinase K
solution (2 mg/ml proteinase K, 1 mM CaCl2–1 mM Tris
pH 7.5) were added to the samples and falcon tubes were
placed overnight in a 37C shaking water bath.