Rotifers that can not be fed immediately need to be stored at a cold temperature (4oC) in
order to prevent the reduction of their nutritional quality. During a starvation period of one
day at 25°C, rotifers can lose up to 26 % of their body weight as a result of metabolic
activity. Different culture and enrichment procedures also influence the effect of starvation.
For example, the starvation of gut-enriched rotifers (i.e., rotifers boosted with oil
emulsions, microparticulated diets or microalgae) immediately before feeding to the
predator (indirect enrichment procedure, short term enrichment) results in a very fast loss
of their fatty acid content, as the animals start to empty their guts after 20 to 30 min! After
about 6 hours in the larval rearing tanks, the rotifer HUFA content may have dropped to
1/3 of its original level. Tissue enrichment (direct enrichment procedure, long term
enrichment), on the other hand takes place during the rotifer culture, and allows a slow but
steady increase in the fatty acid content of the rotifers. This reserve in fatty acids is thus
more stable and less exposed to fast decrease by starvation
Rotifers that can not be fed immediately need to be stored at a cold temperature (4oC) in
order to prevent the reduction of their nutritional quality. During a starvation period of one
day at 25°C, rotifers can lose up to 26 % of their body weight as a result of metabolic
activity. Different culture and enrichment procedures also influence the effect of starvation.
For example, the starvation of gut-enriched rotifers (i.e., rotifers boosted with oil
emulsions, microparticulated diets or microalgae) immediately before feeding to the
predator (indirect enrichment procedure, short term enrichment) results in a very fast loss
of their fatty acid content, as the animals start to empty their guts after 20 to 30 min! After
about 6 hours in the larval rearing tanks, the rotifer HUFA content may have dropped to
1/3 of its original level. Tissue enrichment (direct enrichment procedure, long term
enrichment), on the other hand takes place during the rotifer culture, and allows a slow but
steady increase in the fatty acid content of the rotifers. This reserve in fatty acids is thus
more stable and less exposed to fast decrease by starvation
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