A mode combined with FLP and SCX-SPE cleanup. FLP showed efficient removal of large amounts of lipids extracted from food samples without any sig-nificant loss of PAs. Compared with other cleanup methods for removing lipids, FLP showed easy method to handle and enableto control sample size, and convenient to combine with a SPE car-tridge. Based on the SPE elution patterns of basic PAs on the SCX adsorbent, remaining PAs after the FLP procedure were effectively purified from complicated sample matrix. In view of the recovery and sensitivity of PAs as well as the removal of interferences,the FLP and the SCX-SPE cleanup approach was effective for thedetermination of trace PAs in high lipid foodstuffs.The proposed MS/MS fragmentation pathways of PAs were sug-gested for the confirmation and precise quantification used in theMRM mode. Thus, the use of LC-MS/MS-MRM mode allowed forpositive peak confirmation and the quantification of trace PAsby selecting the precursor-product ions. The developed methodshowed excellent performance in terms of the linearity, accu-racy, precision, recovery, and sensitivity. This established methodwas successfully applied for the determination of trace PAs with