DNA template, and 0.5 U TaKaRa Taq polymerase.
Programmed for an initial 5 min at 94°C, then followed by
35 cycles for 30 s at 94°C, 30 s at 56°C, and 1 min at 72°C,
and finally 10 min at 72°C. The PCR products were separated
on 10% non-denatured PAGE gels and stained with silver
nitrate solution