The nucleotide sequence of Mr-IAG (GenBank Accession no.
FJ409645.1; Ventura et al., 2009), was converted to a peptide
sequence using the Gene Runner program. A conserved hydrophilic
segment (assessed by http://www.expasy.ch/tools/protscale.html)
of 22 amino acids of the B chain (LASVCLRHNNYINPGPTYVSKE) was
selected for synthesis by GenScript Corporation, Piscataway, NJ,
USA. The synthetic peptide was then used for polyclonal antibody
production.