Using the optimum pretreatment conditions obtained from the
previous section (biomass loading = 10% (w/v), catalyst loading = 4%
(w/v), pretreatment time = 30 min, and temperature = 120
◦
C), pre-
treated MCR is subsequently used to investigate the effects of
cellulase concentration on enzymatic hydrolysis of pretreated MCR.
The cellulase concentrations studied are 5, 15, 30, 45 and 60 FPU/g
while the
-glucosidase concentration were maintained constant
at 52 CBU/g for all experiments.