The diluted extract was reacted with Folin–Ciocalteu reagent (Sigma Chemical Co., MO, USA) for 10 min followed with addition of 20% NaCO3 and incubation in a 40 C water bath for 15 min (UV160U, Shimadzu, Japan).
The diluted extract was reacted with Folin–Ciocalteureagent (Sigma Chemical Co., MO, USA) for 10 min followed withaddition of 20% NaCO3 and incubation in a 40 C water bath for15 min (UV160U, Shimadzu, Japan).