Stearic acid
palmitic acid
oleic acid
dissolved
stock
solution
pure
ethanol
concentrations
respectively
ethanol stock solutions
vortex
mixed
minutes and diluted to obtain the desired final
concentration in the culture medium. Before use, all media
were vigorously shaken and filter sterilized under aseptic
conditions. Non-esterified fatty acid solvability was
checked in all NEFA medium samples before exposure to
determine whether the intended concentrations wereactually achieved. Final NEFA concentrations were
measured at the “Algemeen Medisch Labo” (Antwerp,
Belgium), using commercial photometric assays, RX Daytona
(Randox Laboratories). Measurements were carried
out according to the manufacturers’ instructions