the ELISA plate wells were incubated with rabbit antiserum against olive flounder IgM (diluted 1000-fold with PBS containing 5% skim milk) at 25 C for 30 min,
the ELISA plate wells were incubatedwith rabbit antiserum against olive flounder IgM (diluted 1000-foldwith PBS containing 5% skim milk) at 25 C for 30 min,