The half brown rice was affixed on sample
stage, the cross-section of which was upward, then the
cross-section of the half brown rice was gold-plated
by using vacuum coating machine (Hitachi, Japan).
Finally, 20 kV acceleration voltage was added in SEM,
and the observation and taking photos over different
position of the cross-section of the half brown rice
under SEM were conducted (Kang and Cheng, 2007).