Mixture was then cooled to room temperature and centrifuged at10,621g for 5 min and 0.250 ml of the supernatant was diluted with 1 ml of distilled water.
Mixture was then cooled to room temperature and centrifuged at10,621g for 5 min and 0.250 ml of the supernatant was diluted with 1 ml of distilled water.