At least four successive batches were made to each fermentation
medium prior to change to the following medium with increased
inhibitor levels. Adaptation was monitored by calculating
the specific maximum glucose consumption rate of each batch,
measured at 10% or less of the initial glucose remaining in the reactor.
After the CRBF adaptation, the obtained strain (Y-265A3) was
cultivated on YM agar plates supplemented with 1 g/L furfural
and 4 g/L acetic acid for maintenance and further evaluation, since
culture in synthetic inhibitor-containing solid medium (E medium)
proved unfeasible.