Considering the results here obtained,
for the precipitation of
bromelain, the first application (0 min) was set at 0.25 mL (20%
v/v ethanol), at concentrations of ethanol between 20% and 50%
(v/v), the volume was completed to the desired concentration in
the 1st min, and for concentrations above 50% (v/v), the volume
of ethanol was completed in the 2nd min.