Take note of where – and to what extent – the detector output voltage deviates from a straight-line response. This is an important benchmark: Keep the test data on file as part of your equipment validation record, and take care to insure that – during actual operation – injected activities do not result in a ratemeter signal that substantially exceeds this ‘saturation’ level.
Finally, if the ‘saturation’ signal level is too high (or too low) for your chromatography data system, then it will be necessary to re-adjust the detector gain-set trim potentiometer and repeat the above procedure until the maximum expected signal levels are compatible with your data acquisition system.