Extracted date seed oil was purified using a vacuum Rotary evaporator at 40 ?C and ?760 mmHg. Lipase Was immobilized onto calcium alginate beads by mixing 1 g of lipase into 1 g of sodium alginate dissolved in 100ml of water. This mixture was subsequently introduced by using narrow tip burette into 0.1M calcium chloride solution to form calcium alginate beads (0.35 mm).