The white starch residue was resuspended in an aqueous solution of 0.1 M NaCl:toluene (7:1 v/v) and then homogenized and stirred overnight at 4 1C.
The suspension was centrifuged (7000g, 4 1C, 30 min) so that two phases were formed: the proteins, fat and small granules not separated by centrifugation in the toluene phase; and a starch-rich fraction in the aqueous phase.
This fraction was washed repeatedly with a NaCl–toluene mixture.
The isolated starch was dried in a convection oven at 35 1C for 24 h, carefully ground in a mortar and stored at room temperature (25 1C) in airtight plastic bags.