We used freshly cultured U. virens to inoculate rice panicles at the late booting stage, the concentration of inoculum was 107 conidia per milliliter (Figure 1(a)). At day 5 after infection, hyphae emerged and enclosed rice spikelets (Figure 1(b)). Usually, flower-infecting fungi
develop hyphae on the exterior surface of florets and grow toward the openings. Rice spikelets covered by U. virenshyphae were opened up to examine the infection process inside the spikelet (Figure 1(c)). Hyphae entered florets through the aperture ofthe apex of glumes, firstly
occupied the upper space of the flower organs and formed a three-dimensional network (Figure 1(d)), then grew downward and intertwined the stamens and the bifurcate feathery stigma (Figure 1(e)). We observed large amounts of hyphae on the surface of anthers and plumose stigma (Figures 1(f), (g)). Growing downward, hyphae filled the whole insidespace of the floret and enclosed the ovary (Figure 1(h)). This observation is in agreement with the findings of Ashizawa et al. [24] in that they found U. virensinvaded rice spikelets through the small gap of the spikelet apex.
We used freshly cultured U. virens to inoculate rice panicles at the late booting stage, the concentration of inoculum was 107 conidia per milliliter (Figure 1(a)). At day 5 after infection, hyphae emerged and enclosed rice spikelets (Figure 1(b)). Usually, flower-infecting fungi
develop hyphae on the exterior surface of florets and grow toward the openings. Rice spikelets covered by U. virenshyphae were opened up to examine the infection process inside the spikelet (Figure 1(c)). Hyphae entered florets through the aperture ofthe apex of glumes, firstly
occupied the upper space of the flower organs and formed a three-dimensional network (Figure 1(d)), then grew downward and intertwined the stamens and the bifurcate feathery stigma (Figure 1(e)). We observed large amounts of hyphae on the surface of anthers and plumose stigma (Figures 1(f), (g)). Growing downward, hyphae filled the whole insidespace of the floret and enclosed the ovary (Figure 1(h)). This observation is in agreement with the findings of Ashizawa et al. [24] in that they found U. virensinvaded rice spikelets through the small gap of the spikelet apex.
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