Fig. 2. Separation of the DPP IV inhibitory fraction through column chromatography.
A. Separation of a DPP IV inhibitor using RP-HPLC. Four fractions were obtained. B.
Enzyme activities the fractions were measured. Fraction number 2 had inhibitory activity
against DPP IV, and its visible colour was shown to be red. Values are
means ± S.E. n ¼ 4 experiments. Statistically significant: ***p < 0.001. Not significant:
n.s.(For interpretation of the references to colour in this figure legend, the reader is
referred to the web version of this article.)