2.3. Analytical procedures
Tensile properties of the MPPM were determined using testing
machine ‘‘Instron/5569”. The stress–strain curves of the MPPM
were obtained following the standard ISO 2062 (1993).
The biodiesel content in the reaction mixture was analyzed
using a gas chromatograph (Agilent Technologies 6890N model)
equipped with a DB-WAX (length 30 m, inner diameter 0.53 mm,
film thickness 0.5 lm) capillary column and a flame-ionization
detector (FID) connected to an integraph (DIN EN 14103, 2003).
Mono-, di-, tri-glycerides, free and total glycerol determinations
were carried out according to European standard test method of
DIN EN 14105 (2003). All of the analytical assays were performed
in duplicate and mean values were presented.