Extraction of DFLA
DFLA (30 mg) was mixed with the solvent (1.6 mL), and the
mixture was vigorously stirred for 5 minutes. The mixture was
then centrifuged (13,000g, 25 C) for 20 minutes. The supernatant
obtained was concentrated under vacuum at 45C and
freeze-dried for 24 hours. The freeze-dried extract was
weighed to determine the extraction yield. All freeze-dried
extracts were kept at 4C prior to analyses.