Hexane extracts of all the rice bran varieties were obtained
by Soxhlet apparatus. In the oils from individual
varieties, c-oryzanols were determined by a previously
reported reverse phase HPLC method (McBride &
Evans, 1973). HPLC unit model Hitachi L-6200, specifications
are as: column (C 18, 150 · 2.1 mm, 5 lm), UV–
Vis detector at 330 nm, flow rate 1.4 ml/min, Rheodyne
7125 six-port injector, Sample loop 50 ll and mobile
phase was methanol, acetonitrile, dichloromethane and
acetic acid (50:44:3:3 by volume). The total analysis time
was approximately 22 min and c-oryzanol peaks appeared
around retention time of 16–19 min. A Hitachi
Chromatointegrator model D-2500 with a built-in computer
program was used for data handling and quantification.
Concentrations of all the components of
c-oryzanol were calculated individually from the signal
recorder. The total concentration of c-oryzanol was
achieved by summing up concentrations of all the
components.
Hexane extracts of all the rice bran varieties were obtained
by Soxhlet apparatus. In the oils from individual
varieties, c-oryzanols were determined by a previously
reported reverse phase HPLC method (McBride &
Evans, 1973). HPLC unit model Hitachi L-6200, specifications
are as: column (C 18, 150 · 2.1 mm, 5 lm), UV–
Vis detector at 330 nm, flow rate 1.4 ml/min, Rheodyne
7125 six-port injector, Sample loop 50 ll and mobile
phase was methanol, acetonitrile, dichloromethane and
acetic acid (50:44:3:3 by volume). The total analysis time
was approximately 22 min and c-oryzanol peaks appeared
around retention time of 16–19 min. A Hitachi
Chromatointegrator model D-2500 with a built-in computer
program was used for data handling and quantification.
Concentrations of all the components of
c-oryzanol were calculated individually from the signal
recorder. The total concentration of c-oryzanol was
achieved by summing up concentrations of all the
components.
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