Although various biochemical tests were performed, to identify the Actinomycetes up to species level, for proper identification of genera and species of Actinomycetes, besides morphological and physiological properties, various other biochemical properties such as cell wall chemo type, whole-cell sugar pattern, peptidoglycan type, phospholipids type and G+C% of DNA should be determined. The minimum inhibitory concentration (MIC) for the intracellular antimicrobial extracted from S. aureus was 0.0009mg/ml and for extracellular antimicrobial extracted from S. aureus was 0.0037 mg/ml, this showed that the extracellular antimicrobial from S. aureus was more active than from intracellular antimicrobials.