Screening of pectinase producing Bacteria:
Primary screening:
All of the bacterial isolates were tested for production of pectinase enzyme .The media used for primary screening was
pectinase screening agar medium(PSAM).The sterile PSAM plates were prepared and all bacterial isolates were spot inoculated on those
plates.All plates were incubated at 370C for 48 hours. After incubation the strains which are able to utilize pectin as a source of carbon
develop colony on PSAM media and were selected as positive cultures.