NaCl concentration for control culture was set up at 0.5 mM in addition to the trace elements
(standard Hunter’s trace-elements = 1ml/L). The culture
medium for D. salina also included the same nutrient elements but NaCl concentration was set at 1, 1.5 and 2 M.
In this study, sodium bicarbonate was used instead of CO2 injection due to its ease of use.