In order to stimulate efficient micropropagation of PLB, much effort has been directed to modify the culture media, mainly by the inclusion of plant growth regulators such as N6-benzylaminopurine and a-naphthaleneacetic acid [18], benzyladenine, gibberellic acid and 3- indoleacetic acid [19], various polyamines [20], N6-benzyladenine and thidiazuron [21,22].