Fermented bamboo shoot is one of the fermented food in the south of Thailand. This product can be stored for a year or more. The problem of the product
was film yeast on the surface of fermented solution that the consumers dislike after opening the container. The yeast occurred in samples of liquid at the surface of fermented bamboo shoot product from villagers in
Pattani Province. Many species of yeasts were identified as Saccharomyces cerevisiae J1, Candida krusei J2 and C. krusei J3. All species tolerated 2.5% NaCl addition (1). Garcia et al. (2) reported that in the presence of 1 M NaCl showed a reduction in growth rate and adaptation of yeasts. Because yeast has an adaptative mechanism to increase sodium efflux or restrict sodium uptake during
788
KKU Res. J. 2012; 17(5)
continuous growth in media with NaCl. Yeast can defend against salt stress by the osmolytes such as Glycerol. Also know as ‘budding yeast’, is able to undergo a morphology to produce filaments of elongated cells in diploids and invasive filaments in haploids (3). But the switch from budding to filamentous has been found in the pathogen (e.g., Candida albicans). Dickinson (4) discovered ‘fusel’ alcohols induce hyphal-like extensions and pseudohyphal formation in yeasts. Fusel alcohols are the products of amino acid catabolism which accumulate
under nutrients limitation (4,5). While much of work carried out to date in relation to food preservation was using chemical treatments such as benzoic acid and sorbic acid, or adding salt solution to inhibit the growth of film yeasts. A tolerance for the preservatives were a one problems for the food industry. It need to increasing the levels to prevent yeast spoilage (6). In addition, C. krusei spoilage is often characterization to tolerate the conditions in foods, the fermentation of available sugars can produce CO2 and leading to bloating (7,8). Other, there is considerable for possibility to relation. Therefore, the focus of our research was to study the morphology and physiology of film yeast.
Fermented bamboo shoot is one of the fermented food in the south of Thailand. This product can be stored for a year or more. The problem of the productwas film yeast on the surface of fermented solution that the consumers dislike after opening the container. The yeast occurred in samples of liquid at the surface of fermented bamboo shoot product from villagers inPattani Province. Many species of yeasts were identified as Saccharomyces cerevisiae J1, Candida krusei J2 and C. krusei J3. All species tolerated 2.5% NaCl addition (1). Garcia et al. (2) reported that in the presence of 1 M NaCl showed a reduction in growth rate and adaptation of yeasts. Because yeast has an adaptative mechanism to increase sodium efflux or restrict sodium uptake during788KKU Res. J. 2012; 17(5)continuous growth in media with NaCl. Yeast can defend against salt stress by the osmolytes such as Glycerol. Also know as ‘budding yeast’, is able to undergo a morphology to produce filaments of elongated cells in diploids and invasive filaments in haploids (3). But the switch from budding to filamentous has been found in the pathogen (e.g., Candida albicans). Dickinson (4) discovered ‘fusel’ alcohols induce hyphal-like extensions and pseudohyphal formation in yeasts. Fusel alcohols are the products of amino acid catabolism which accumulateunder nutrients limitation (4,5). While much of work carried out to date in relation to food preservation was using chemical treatments such as benzoic acid and sorbic acid, or adding salt solution to inhibit the growth of film yeasts. A tolerance for the preservatives were a one problems for the food industry. It need to increasing the levels to prevent yeast spoilage (6). In addition, C. krusei spoilage is often characterization to tolerate the conditions in foods, the fermentation of available sugars can produce CO2 and leading to bloating (7,8). Other, there is considerable for possibility to relation. Therefore, the focus of our research was to study the morphology and physiology of film yeast.
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