530 nm was lower than that of f3h (Figure 5B). However, the fresh red extract of the abscission 1 DPA petal and the corresponding increase in OD value at 530 nm indicated that the increase in red color of the developing flower was due to anthocyanin. F3H silencing line had the similar color changes as previously described for non-abscission 1 DPA petals [46]. These results confirmed that flavonoids are the main pigment responsible for cotton flower color including red, cream and white.