2. Determination of Vitamin A
Reagents:
1. Mixed solution of n-hexane : acetone in the ratio of 1:1
2. Sodium sulfate anhydrous
3. Standard -carotene
Procedure:
1. Add 15 ml of non-clarified juice in separation funnel.
2. Add 45 ml of n-hexane : acetone 1:1.
3. Shake for 1 min and hold for 5 minutes to allow separation of phase.
4. Collect solvent phase and keep in beaker.
5. Repeat extraction by following the procedure No. 2-4.
6. Add sodium sulfate anhydrous (5 g) and filtrate by Whatman No.1 paper.
7. Measure the absorbance at 450 nm by spectrophotometer and set zero with solvent.
8. Calculate the -carotene concentration by standard