This research study of titanium dioxide thin films prepared by RF techniques sputtering sterling on glass slides. To study the inhibitory properties e.coli of a thin film of titanium dioxide (tio2) conditions at different sintering temperatures. Then the film samples were prepared for analysis of crystal structures. Surface characteristics and properties absorbance xrd and sem techniques and uv-visible.And testing photocatalysis Sea of film by considering the types of bacteria e.coli with UV light. The results of the study revealed that the film is structured phase Anaheim synthase and room Tide and study the surface to find that the surface characteristics of thin film thickness of 10 nm and 100 nm were found to be the ground. smooth100 nm calcined at 450 ° C for 2 h, bacteria were 87% in 120 minutes.