Preparation of antagonistic fungal isolates
Isolates of three fungal antagonists including T. flavus,
T. harzianum, and T. asperellum were obtained from the research
laboratory’s microbial collection of beneficial microorganisms,
of the Iranian Research Institute of Plant
Protection. In 2013, these isolates were isolated from the
soil of garlic and potato fields located in the province of
Hamedan, Iran. The antagonistic activities of the isolates
against some fungal pathogens, including S. cepivorum,
were previously evaluated and approved in in vitro conditions
in the above-mentioned laboratory (unpublished
data). The antagonistic fungal isolates used in the study
and their characteristics are presented in table 1.