Glucose oxidase (GOx)has found a wide range of applications in chemical, food, beverage, biotechnology and other industries. Previously we have reported that the production of GOx by a newly isolated Aspergillus terreus UniMAP AA- I was largely determined by Glucose, NaNO3 and CaCO3 as identified by Plackett-Burman design.In the present paper,the influences of those elements were further investigated with one-factor-at-a-time (OFAT). The result shows that the optimum GOx production was achieved at 4.0%, 1.0% (w/v)for CaCO,NaNO and glucose respectively