To determine the total phenolic content of FPP
(1:1 kg/kg) was prepared. 1 ml of juice (fresh
grounded pumpkin or reconstituted puree powder)
was taken into a test tube and Folin-Ciocalteu's
(Sigma-Aldrich Corp.) reagent (5 ml) that diluted
10- fold before use and 2% Na2CO3
(15 ml) were
added. After reaction for 2 hours, absorbance was
measured at 760 nm. A calibration curve of gallic
acid (3, 4, 5-trihydroxybenzoic acid) (Sigma-Aldrich
Corp.) was prepared. The results were given as
gallic acid equivalents per grams of sample (24).