The stool samples were collected into a disposable plastic
container and then delivered immediately to the laboratory
for examination. Fresh fecal samples were analyzed for the
presence of cysts or trophozoite in the feces by microscopic
examination, using the sucrose gradient concentration
method. After staining with Lugol’s solution, samples
were also examined by ELISA test (Dahlewitz, Berlin,
Germany). The age, gender, living place and symptoms
of subjects were also recorded. Data were analyzed using
the Statistical Package for the Social Sciences software
program. The Chi-square test was used for assessment of
relation of Giardia infestation and clinical symptoms. P
value less than 0.05 was considered statistically significant.
Statistical kappa agreement rate was used for direct stool
examination and ELISA test agreement rate. A kappa value
of 1 indicates prefect agreement and zero shows that the
agreement is not better than chance.