A fermentation medium with varying pH values (2–7) was used to moist the growth supported substrate, lemon peel waste, for the production of pectin lyase. The maximum activity 618 ± 8.98 U/mL was recorded in the SSF media containing lemon peel waste processed at pH 5. From the growth pattern of fungus and culture extracts it was observed that the enzyme formation progressively increased with an increase in the pH of fermentation media and maximum activity was recorded at pH 5 (Fig. 3)