The fully developed plantlets were in-vitro hardened for 25
days in half strength MS media, then transferred to different
potting media under Green House conditions. After 30 days,
the plantlets in the different potting media were analyzed for
percentage of survival and average increase in height. Silva et
al. (1998) have also successfully pre-acclimatized the rooted
plantlets of cv. Pioneira for 30 days in a Green House under
controlled conditions of temperature and irrigation.