The medium for microbial cultivation was prepared using a nutrient rich solution containing: glucose, 50 (g/L); yeast extract,5 (g/L); (NH4)2SO4, 7.5 (g/L); MgSO4•7H2O, 0.75 (g/L); K2HPO4, 3.5(g/L); and CaCl2•2H2O, 1(g/L) at pH 5.5 ± 0.1. The medium (100 ml) was added to a 250 ml cotton plugged Erlenmeyer and autoclaved at 121◦C for 20 min.