Plant Material and Extraction
The leaves of A. princeps were obtained from the oriental drug store Dae Hak Yak Kuk (Iksan, South Korea). The identity of the specimen was confirmed by Young-Hoi Kim at the College of Environmental and Bioresource sciences, Chonbuk National University (Jeonju, South Korea). A voucher specimen (number 8-10-13) has been deposited at the Herbarium of the Department of Oral Biochemistry in Wonkwang University. Dried leaves (250 g) of A. princeps were soaked in 3,500 mL of 70% ethanol for 72 h at room temperature. The extracted solution was filtered and evaporated under reduced pressure to yield an ethanol extract of 5.7 g (2.28%). After the extract was thoroughly dried to facilitate complete removal of the solvent, the dry extract was dissolved in DMSO to give the desired stock solution. The final concentration of DMSO applied to culture systems was adjusted to 0.1% (v/v), which did not interfere with the testing system. Control groups were treated with media containing 0.1% DMSO.
Plant Material and ExtractionThe leaves of A. princeps were obtained from the oriental drug store Dae Hak Yak Kuk (Iksan, South Korea). The identity of the specimen was confirmed by Young-Hoi Kim at the College of Environmental and Bioresource sciences, Chonbuk National University (Jeonju, South Korea). A voucher specimen (number 8-10-13) has been deposited at the Herbarium of the Department of Oral Biochemistry in Wonkwang University. Dried leaves (250 g) of A. princeps were soaked in 3,500 mL of 70% ethanol for 72 h at room temperature. The extracted solution was filtered and evaporated under reduced pressure to yield an ethanol extract of 5.7 g (2.28%). After the extract was thoroughly dried to facilitate complete removal of the solvent, the dry extract was dissolved in DMSO to give the desired stock solution. The final concentration of DMSO applied to culture systems was adjusted to 0.1% (v/v), which did not interfere with the testing system. Control groups were treated with media containing 0.1% DMSO.
การแปล กรุณารอสักครู่..
