One of the main purposes of modern agriculture is to raise crop production by means of a proper
application of herbicides. Nonetheless, the use of herbicides is causing some concerns associated to their
persistence and accumulation in the environment. Notwithstanding the relevance of these aspects, little
or no attention has been paid to the interferences that these chemicals and their residues can exert on
iron (Fe) mineral nutrition of plants. To this purpose, we studied the effect of terbuthylazine (TBA) on the
Fe-acquisition processes of Fe-deficient barley plants, by investigating some aspects related to
phytosiderophores (PS) exudation and sulfur (S) metabolism. Results showed that plant growth,
chlorophyll content expressed as SPAD index, PS release and the expression of genes involved in their
secretion, uptake and biosynthesis were negatively affected. This response was associated with reduced
cysteine concentrations, and it suggests that the TBA interferences on Fe-acquisition in barley are the
consequence of the induced changes in S metabolism.