Purified cultures were streaked onto CaCO3-agar plates to
confirm acid production and investigate overoxidation of
acetic acid by formation and disappearance of clear zones
around colonies [1]. CaCO3-agar medium was composed of
0.05% D-glucose, 0.3% peptone, 0.5% yeast extract, 1.5%
CaCO3, 1.2% agar and 1.5%(v/v) ethanol. Microbial growth
was examined during incubation at 30ºC for 2-7 days.