Bonferroni correction was used to adjust for multiple comparisons (Table 3). A Receiver Operating Characteristic (ROC) curve [26] comparing different urine treatment methods in the urine OV-ES assay was constructed by plotting the sensitivity against 1-specificity for the OD values detected using each urine treatment method on FECT confirmed positives and negatives. The Area under the Curve (AUC) was a measure used to determine the probability of correctly identifying an OV positive individual (as determined by FECT) as a True Positive and an OV negative individual (as determine by FECT) as a True Negative. The urine treatment methods that generated the ROC curve with the highest AUC was considered the optimal urine treatment method for use in the urine OV-ES assay.