An easy-to-use method for quantitative analysis of bacterial exopolysaccharides (EPS) was developed.
The acid treatment was resultant in removing proteins and lactose and allowed to avoid long-term operation
as dialysis. It was shown that, as opposed to anthron and phenol–sulphuric acid methods, reducing
sugars method after EPS hydrolysis with glucose as standard provided reliable results, no matter to the
EPS monosaccharide composition.
EPS quantity in kefir of different brands was varied greatly, approximately at 50–200 mg/l, showing
potential to enhance kefiran production by alteration of technological process. The temperature optimum
for EPS production by kefir grains and kefir starter was about 30 C, while the rise of temperature
increased soluble EPS in culturing media by means of extraction from kefir grain body.
An easy-to-use method for quantitative analysis of bacterial exopolysaccharides (EPS) was developed.The acid treatment was resultant in removing proteins and lactose and allowed to avoid long-term operationas dialysis. It was shown that, as opposed to anthron and phenol–sulphuric acid methods, reducingsugars method after EPS hydrolysis with glucose as standard provided reliable results, no matter to theEPS monosaccharide composition.EPS quantity in kefir of different brands was varied greatly, approximately at 50–200 mg/l, showingpotential to enhance kefiran production by alteration of technological process. The temperature optimumfor EPS production by kefir grains and kefir starter was about 30 C, while the rise of temperatureincreased soluble EPS in culturing media by means of extraction from kefir grain body.
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