was mixed with 30 nM fluorescein (150 ll) and
with 38.5 mM AAPH (25 ll). The fluorescence was measured at
excitation of 485 nm and at emission of 520 nm (1.5 h; 37 C) in
a spectrofluorometer Synergy HT (Biotek Instruments, Winooski,
VT, USA). The area under the curve (AUC) and the net AUC of the
Trolox standards (0–2.5 lg/ml) and extracts were obtained by
using the software Gen5 (Biotek Instruments).