All collected substrates were chopped into small pieces (2-4 cm long) and soaked in tap water for overnight. The
excess water in the substrates was allowed to run off until set to required moisture. Each substrate was separately
supplemented with 10 % chicken manure or rice bran and mixed thoroughly; substrate without supplement was
considered as a control. Finally, moisture of each treatment was set to 60% (±2). Following this treatment, the wet
substrates weighing 1 kg were placed in the polypropylene bags of 25 cm 15 cm size and sterilized at 121oC for 1 hr.
After cooling, each bag was inoculated with 2.5% (w/w) spawn under aseptic conditions by placing the spawn on
the substrate surface through the opening at the top of the bag. The inoculated bags were incubated at an ambient
temperature of 25oC (±2) and spawn run (mycelia extension) was observed regularly until appears white
(colonization).