GC–MS analyses of ethyl carbamate were performed by selected
ion monitoring of m/z 62. The retention time of EC was between
13.4 and 13.6 min. During sugar-cane juice fermentation, EC values
changed from zero in the sugar-cane juice to a maximum level of
160 mg L1 in wine (after 24 h of fermentation) as shown in
Fig. 2. Average EC value after fermentation period from three repetitions
was 122 mg L1.