A preliminary screening to determine the การเปลี่ยนแป้งเป็นน้ำตาล activity of the bacteria strains was performed through pour plate technique. Each bacteria strain was inoculated separately at the concentration of 6 log CFU·mL− 1 in MRS agar (de Man–Rogosa–Sharpe) containing 20 g of soluble starch. L− 1 (Sigma, ACS reagent 9005-84-9) as the carbon source. The petri dishes were maintained at 37 °C during 24 h in aerobiosis for L. แพลนทารั่ม A6 and L. เฟอเม็นตุม Ogi E1 and during 72 h in anaerobiosis provided by Anaerocult® A (Merck, Darmstadt, Germany) for the probiotic strains. Afterwards, the colonies were enumerated and then, the culture plates were covered with some drops of Lugol iodine and the colonies with halo of starch hydrolysis were counted (Sanni, Morlon-Guyot, & Guyot, 2002). The results were expressed as % of colonies presenting amylolytic activity.